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Image Search Results
Journal: PLoS ONE
Article Title: Novel Gene Therapy Viral Vector Using Non-Oncogenic Lymphotropic Herpesvirus
doi: 10.1371/journal.pone.0056027
Figure Lengend Snippet: A: Growth curves for wtHHV-6 and H6R28LEP in peripheral blood mononuclear cells (PBMCs). PBMCs were infected with wtHHV-6 virus or H6R28LEP at a multiplicity of infection (MOI) of 1. Culture supernatant was collected every 3 d, and 1×10 6 fresh PBMCs were added after each collection. Progeny viruses were titered on MT4 cells by using immunofluroescent assays (wtHHV-6) or by counting EGFP-positive cells (H6R28LEP). Virus titers are indicated in focus-forming units per milliliter (FFU/ml). B: Growth curves for H6R28LEP in PBMCs and cord blood mononuclear cells (CBMCs). PBMCs and CBMCs were infected with H6R28LEP, and progeny viruses were titered every 3 d as described for panel A. C: Replication of viral DNA in PBMCs. PBMCs were infected with H6R28LEP as described, and infected cells were harvested at the indicated times and frozen at −80°C. Infected cells then were incubated with lysis buffer containing 1 mg/ml proteinase K, and the copy numbers of H6R28LEP in cell lysates were quantified by real-time PCR using primers specific for EGFP. Results are given as mean ±1 standard deviation ( n = 3).
Article Snippet: For the titration of recombinant viruses,
Techniques: Infection, Incubation, Lysis, Real-time Polymerase Chain Reaction, Standard Deviation
Journal: eLife
Article Title: MARCH8 inhibits viral infection by two different mechanisms
doi: 10.7554/eLife.57763
Figure Lengend Snippet:
Article Snippet: Cell line ( H. sapiens ) ,
Techniques: Recombinant, Sequencing, Transfection, Enzyme-linked Immunosorbent Assay, Luciferase, Immunoprecipitation, Protease Inhibitor, Western Blot, Immunofluorescence, Software